Catalog Number - 65508-05P-2

Zx Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octyl (C8) bonded phase. Designed as a cost-effective alternative to Agilent Zorbax® C8 columns, it offers comparable chromatographic behavior for USP L7 methods while providing the moderate hydrophobic retention, reproducibility, and reliability expected from a conventional C8 stationary phase. Compared to C18 columns, C8 phases generally provide reduced retention and faster elution of hydrophobic analytes.

65508-05P-2 is a 50 mm x 2.1 mm ID narrow-bore analytical column packed with 5 µm Zx Similars C8 particles. The short 50 mm length is optimized for rapid analyses and reduced solvent consumption, while the 2.1 mm ID format is particularly well suited for LC-MS applications. The 5 µm particle size provides excellent robustness, broad method compatibility, and lower operating pressure than smaller particle columns. Typical operating flow rates are approximately 0.2–0.3 mL/min and require an HPLC or LC-MS system capable of reliable low-flow operation with low-volume plumbing and flow cells.

This USP L7 column is ideal for hydrophobic and neutral compounds when less retention is desired than a comparable C18 phase. Its narrow-bore design, low-flow operation, and fast analysis capability make it a popular choice for LC-MS methods, pharmaceutical analysis, long-chain fatty acid separations, and high-sensitivity analytical workflows requiring chromatographic behavior similar to Agilent Zorbax® C8 columns.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L7 methods

• Comparable selectivity to Agilent Zorbax® C8 columns

• Narrow-bore format ideal for LC-MS applications

• Fast analysis with 50 mm column length

• Lower operating pressure than smaller particle columns

• Reduced solvent consumption

• End-capped C8 bonded phase

Typical Applications

• USP L7 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Quality control testing

• Fast analytical separations

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Zx Similars C8 Octyl

• USP Classification: L7

• Particle Size: 5 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C8 (Octyl)

• Endcapped: Yes

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Lower operating pressure than smaller particle columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Provides less retention than comparable C18 stationary phases

• Excellent alternative for many USP L7 methods

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